Osteoarthritis (OA) yana daya daga cikin cututtukan haɗin gwiwa na kasusuwa na yau da kullum wanda ke shafar tsofaffi fiye da 65.
1]. Gabaɗaya, ana bincikar marasa lafiya na OA tare da lalatawar guringuntsi, kumburin synovium, da lalata chondrocytes, waɗanda ke haifar da ciwo da damuwa ta jiki [
2]. Ciwon arthritic yawanci yana haifar da lalacewa na guringuntsi a cikin gidajen abinci ta hanyar kumburi, kuma lokacin da guringuntsi ya lalace sosai kasusuwa na iya yin karo da juna yana haifar da ciwo maras iya jurewa da wahala ta jiki [
3]. Shigar da masu shiga tsakani masu kumburi tare da bayyanar cututtuka irin su ciwo, kumburi, da taurin haɗin gwiwa an rubuta su sosai. A cikin marasa lafiya na OA, cytokines masu kumburi, waɗanda ke haifar da yashwar guringuntsi da ƙashi na subchondral ana samun su a cikin ruwan synovial [
4]. Manyan gunaguni guda biyu waɗanda marasa lafiyar OA gabaɗaya ke da su sune zafi da kumburin synovial. Saboda haka maƙasudin farko na hanyoyin kwantar da hankali na OA na yanzu shine don rage ciwo da kumburi. [
5]. Ko da yake akwai jiyya na OA, ciki har da wadanda ba steroidal da steroidal kwayoyi, sun tabbatar da tasiri a rage zafi da kumburi, da dogon lokacin amfani da wadannan kwayoyi suna da mummunan sakamako na kiwon lafiya irin su zuciya da jijiyoyin jini, gastro-na hanji, da kuma renal dysfunctions.
6]. Don haka, dole ne a samar da magani mafi inganci tare da ƙarancin illa don maganin osteoarthritis.
Samfuran kiwon lafiya na halitta suna ƙara shahara don kasancewa cikin aminci da sauƙin samuwa [
7]. Magungunan gargajiya na Koriya sun tabbatar da inganci a kan cututtukan kumburi da yawa, gami da arthritis [
8]. Aucklandia lappa DC. an san shi da kayan magani, kamar haɓaka zagayawa na qi don kawar da ciwo da sanyaya cikin ciki, kuma an yi amfani dashi a al'ada azaman analgesic na halitta [
9]. Rahotanni na baya sun nuna cewa A. lappa yana da maganin kumburi [
10,
11analgesic [
12anticancer [
13da kuma gastroprotective.
14] illa. Ayyukan nazarin halittu daban-daban na A. lappa suna lalacewa ta hanyar manyan abubuwan da ke aiki: costunolide, dehydrocostus lactone, dihydrocostunolide, costuslactone, α-costol, saussurea lactone da costuslactone.
15]. Nazarin da suka gabata sun yi iƙirarin cewa costunolide ya nuna kaddarorin anti-mai kumburi a cikin lipopolysaccharide (LPS), wanda ya haifar da macrophages ta hanyar ka'idar NF-kB da hanyar furotin mai zafi.
16,
17]. Koyaya, babu wani binciken da ya bincika yuwuwar ayyukan A. lappa don maganin OA. Binciken da aka yi a yanzu ya binciki tasirin warkewa na A. lappa akan OA ta amfani da (monosodium-iodoacetate) MIA da acetic acid-induced rodent model.
Monosodium-iodoacetate (MIA) sanannen sananne ne don samar da yawancin halayen raɗaɗi da sifofin pathophysiological na OA a cikin dabbobi.
18,
19,
20]. Lokacin da aka yi masa allura a cikin haɗin gwiwa, MIA yana lalata metabolism na chondrocyte kuma yana haifar da kumburi da alamun kumburi, irin su guringuntsi da yashwar ƙashi na ƙasa, alamun alamun OA.
18]. Amsar amsa da aka jawo tare da acetic acid ana ɗaukarsa sosai azaman simintin ɓacin rai a cikin dabbobi inda za'a iya auna ciwon kumburi da ƙima.
19]. Layin macrophage na linzamin kwamfuta, RAW264.7, ana amfani da shi sosai don nazarin martanin salon salula ga kumburi. Bayan kunnawa tare da LPS, RAW264 macrophages suna kunna hanyoyi masu kumburi da ɓoye da yawa masu tsaka-tsakin kumburi, kamar TNF-α, COX-2, IL-1β, iNOS, da IL-6.
20]. Wannan binciken ya kimanta tasirin anti-nociceptive da anti-mai kumburi na A. lappa a kan OA a cikin samfurin dabba na MIA, samfurin dabba na acetic acid, da LPS-activated RAW264.7 Kwayoyin.
2. Kayayyaki da Hanyoyi
2.1. Kayan Shuka
Busashen tushen A. lappa DC. da aka yi amfani da shi a cikin gwajin an samo su daga Epulip Pharmaceutical Co., Ltd., (Seoul, Korea). Farfesa Donghun Lee, Dept. of Herbal Pharmacology, Col. na Korean Medicine, Gachon University ne ya gano shi, kuma an ajiye lambar samfurin bauchi a matsayin 18060301.
2.2. Binciken HPLC na A. Lappa Extract
An ciro A. lappa ta amfani da na'urar reflux (ruwa mai narkewa, 3 h a 100 ° C). Maganin da aka fitar an tace kuma an haɗa shi ta hanyar amfani da ƙarancin matsi. A. Lappa tsantsa yana da yawan amfanin ƙasa 44.69% bayan daskare-bushe a ƙarƙashin -80 °C. An gudanar da bincike na Chromatographic na A. lappa tare da haɗin HPLC ta amfani da tsarin 1260 InfinityⅡ HPLC-tsarin (Agilent, Pal Alto, CA, USA). Don rabuwar chromatic, an yi amfani da shafi EclipseXDB C18 (4.6 × 250 mm, 5 µm, Agilent) a 35 °C. Jimlar 100 MG na samfurin an diluted a cikin 10 ml na 50% methanol da sonicated na 10 min. An tace samfurori tare da tace sirinji (Waters Corp., Milford, MA, Amurka) na 0.45 μm. Tsarin lokaci na wayar hannu shine 0.1% phosphoric acid (A) da acetonitrile (B) kuma an ƙaddamar da ginshiƙi kamar haka: 0-60 min, 0%; 60-65 min, 100%; 65-67 min, 100%; 67-72 min, 0% kaushi B tare da adadin kwarara na 1.0 ml/min. An lura da zubar da ruwa a 210 nm ta amfani da ƙarar allura na 10 μL. An gudanar da bincike a cikin sau uku.
2.3. Gidajen Dabbobi da Gudanarwa
Male Sprague – Dawley (SD) berayen da ke da shekaru 5 makonni da kuma mice ICR masu shekaru 6 makonni an siyi su daga Samtako Bio Korea (Gyeonggi-do, Korea). An ajiye dabbobi a cikin daki ta amfani da zafin jiki akai-akai (22 ± 2 ° C) da zafi (55 ± 10%) da kuma yanayin haske / duhu na 12/12 h. Dabbobin sun san yanayin fiye da mako guda kafin a fara gwajin. Dabbobi suna da wadatar abinci da ruwa ad libitum. Dokokin da'a na yanzu don kula da dabbobi da kulawa a Jami'ar Gachon (GIACUC-R2019003) an bi su sosai a cikin duk hanyoyin gwaji na dabba. An tsara binciken da makafi mai bincike da gwaji mai kama da juna. Mun bi hanyar euthanasia bisa ga ka'idodin Kwamitin Da'a na Gwajin Dabbobi.
2.4. MIA Allurar da Jiyya
An raba berayen bazuwar zuwa ƙungiyoyi 4, wato sham, control, indomethacin, da A. lappa. Kasancewa da anesthetized tare da 2% isofluorane O2 cakuda, an yi amfani da berayen ta amfani da 50 μL na MIA (40 mg / m; Sigma-Aldrich, St. Louis, MO, Amurka) a cikin haɗin gwiwar gwiwa don kaiwa ga gwaji OA. An gudanar da jiyya kamar yadda ke ƙasa: sarrafawa da ƙungiyoyin sham kawai ana kiyaye su tare da abinci na asali na AIN-93G. Kawai, an ba da rukunin indomethacin tare da indomethacin (3 mg / kg) wanda aka haɗa a cikin abinci na AIN-93G kuma an sanya rukunin A. An ci gaba da jiyya har tsawon kwanaki 24 tun daga ranar shigar da OA a cikin adadin 15-17 g ta 190-210 g nauyin jiki a kowace rana.
2.5. Ma'aunin Jikin Nauyi
Bayan shigar da OA, an yi ma'aunin ƙarfin ɗaukar nauyi na gaɓoɓin baya na berayen tare da rashin iyawa-MeterTester600 (IITC Life Science, Woodland Hills, CA, USA) kamar yadda aka tsara. An ƙididdige rabon nauyi akan gaɓoɓin hind: ƙarfin ɗaukar nauyi (%)
