Pure Artemisia capillaris man don kyandir da sabulu yin jumloli diffuser sabon mai sabon mai don masu ƙona reed

Pure Artemisia capillaris man don kyandir da sabulun yin jumhuriyar diffuser muhimmin mai sabo don masu ƙonawar reed Featured Image

taƙaitaccen bayanin:

Tsarin ƙirar rodent

An raba dabbobin zuwa rukuni biyar na beraye goma sha biyar kowanne. Ƙungiya mai sarrafawa da ƙungiyar ƙirar beraye an lalata su daman sesamena kwanaki 6. An yi amfani da berayen ƙungiyar kulawa mai kyau tare da allunan bifendate (BT, 10 mg/kg) na kwanaki 6. An bi da ƙungiyoyin gwaji tare da 100 mg / kg da 50 mg / kg AEO narkar da su a cikin man sesame na kwanaki 6. A rana ta 6, an shayar da rukunin kulawa da man sesame, kuma duk sauran rukunin an bi da su tare da kashi ɗaya na 0.2% CCl4 a cikin man sesame (10 ml/kg) taallurar intraperitoneal. An yi azumin berayen ba tare da ruwa ba, kuma an tattara samfuran jini daga tasoshin retrobulbar; An tattara jinin da aka tattara a 3000 ×gdon 10 min don raba maganin.Ragewar mahaifaan yi shi nan da nan bayan an cire jini, kuma an cire samfuran hanta da sauri. An adana wani ɓangare na samfurin hanta nan da nan a -20 ° C har sai an yi nazari, kuma an cire wani sashi kuma an gyara shi a cikin 10%formalinmafita; sauran kyallen takarda an adana su a -80 ° C don nazarin histopathological.Wang et al., 2008,Hsu et al., 2009,Nie et al., 2015).

Auna ma'auni na biochemical a cikin jini

An kiyasta raunin hanta ta hanyar kimantawaenzymatic ayyukana maganin ALT da AST ta amfani da kayan kasuwanci masu dacewa bisa ga umarnin kayan aikin (Nanjing, Lardin Jiangsu, China). Ayyukan enzymatic an bayyana su azaman raka'a a kowace lita (U/l).

Ma'auni na MDA, SOD, GSH da GSH-Pxa cikin hanta homogenates

An daidaita ƙwayoyin hanta tare da saline mai sanyi mai sanyi a cikin rabo na 1: 9 (w / v, hanta: saline). Homogenates sun kasance a tsakiya (2500 ×gdon 10 min) don tattara masu girma don ƙaddarar gaba. An kimanta lalacewar hanta bisa ga ma'aunin hanta na matakan MDA da GSH da kuma SOD da GSH-P.xayyuka. Duk waɗannan an ƙaddara su bisa ga umarnin kan kayan (Nanjing, Lardin Jiangsu, China). Sakamakon MDA da GSH an bayyana su azaman nmol ta kowace MG protein (nmol / mg prot), da ayyukan SOD da GSH-P.xAn bayyana su azaman furotin U ta MG (U/mg prot).

Histopathological bincike

An gyara sassan hanta da aka samo a cikin 10% bufferedparaformaldehydemaganin phosphate. Sa'an nan kuma an saka samfurin a cikin paraffin, a yanka a cikin sassan 3-5 μm.hematoxylinkumaeosin(H&E) bisa ga daidaitaccen tsari, kuma a ƙarshe an bincika tahaske microscope(Tian et al., 2012).

Binciken kididdiga

An bayyana sakamakon a matsayin ma'anar ± daidaitaccen karkata (SD). An yi nazarin sakamakon ta amfani da shirin ƙididdiga na SPSS Statistics, sigar 19.0. An ƙaddamar da bayanan don nazarin bambancin (ANOVA,p<0.05) sannan gwajin Dunnett da gwajin T3 na Dunnett don tantance bambance-bambance masu mahimmanci tsakanin ƙimar ƙungiyoyin gwaji daban-daban. An yi la'akari da bambanci mai mahimmanci a matakinp<0.05.

Sakamako da tattaunawa

Abubuwan da ke cikin AEO

Bayan nazarin GC/MS, an gano AEO yana ƙunshe da abubuwa 25 waɗanda aka ɓoye daga 10 zuwa 35 min, kuma an gano abubuwan 21 waɗanda ke lissafin kashi 84% na mahimman mai.Tebur 1). Man da ke ƙunshe da maras tabbasmonoterpenoids(80.9%), sesquiterpenoids (9.5%), cikakken hydrocarbons marasa rassa (4.86%) da nau'in acetylene (4.86%). Idan aka kwatanta da sauran karatu (Guo et al., 2004), mun sami monoterpenoids masu yawa (80.90%) a cikin AEO. Sakamakon ya nuna cewa mafi yawan abubuwan da ke cikin AEO shine β-citronellol (16.23%). Sauran manyan abubuwan AEO sun haɗa da 1,8-cineole (13.9%),kafur(12.59%),linalool(11.33%), α-pinene (7.21%), β-pinene (3.99%),thymol(3.22%), kumamyrcene(2.02%). Bambance-bambancen da ke tattare da sinadarai na iya kasancewa yana da alaƙa da yanayin muhallin da shuka ya fallasa, kamar ruwan ma'adinai, hasken rana, matakin ci gaba da haɓakawa.abinci mai gina jiki.

Farashin FOB:US $0.5 - 9,999 / yanki

Min. Yawan oda:Guda 100/Kashi

Ikon bayarwa:10000 Pieces/Perces per month

Aika mana imel

Cikakken Bayani

Tags samfurin

Cutar hanta, cuta ta gama gari da ke haifar da itaviral hepatitis, shaye-shaye, sinadarai masu guba na hanta, halayen abinci mara kyau da gurɓataccen muhalli, damuwa ce ta duniya (Papay et al., 2009). Duk da haka, maganin likita don wannan cuta sau da yawa yana da wuyar gudanarwa kuma yana da tasiri mai iyaka. Sinawa na gargajiyamagungunan ganye, wanda ke ƙarƙashin takaddun magunguna da yawa da ake amfani da su don magance cututtukan hanta, har yanzu Sinawa suna amfani da su sosai (Zhao et al., 2014).Artemisia capillarisBabban.,Asteraceae, bisa ga Bencao Gangmu, sanannun bayanan magungunan gargajiya na kasar Sin, an yi amfani da su sosai a matsayin magani don kawar da zafi, ingantawa.diuresisda cire jaundice kuma an yi amfani da shi azaman dandano a cikin abubuwan sha, kayan lambu, da pastries saboda ƙamshinsa na musamman.A. capillarisyawan jama'a sun ɗauke shi azaman nau'in magani da abinci na jama'ar kasar Sin. Don haka, an yi yunƙurin samar da magungunan ganye masu amfani, kamarA. capillaris, domin maganin ciwon hanta.

A cikin 'yan shekarun nan, magungunan ganye sun sami ƙarin kulawa da kuma shahara don maganin cututtukan hanta saboda amincin su da inganci (Ding et al., 2012).A. capillarisan tabbatar da cewa yana da kyakkyawan aikin hana hanta bisa ga hanyoyin zamani na zamani (pharmacoology).Han et al., 2006). Har ila yau, abu ne mai mahimmanci na magani a kasar Sin kuma sanannen maganin kumburi ne (Cha et al., 2009a),choleretic(Yoon da Kim, 2011), da kuma anti-tumor (Feng et al., 2013)maganin ganye.

PhytochemicalBincike ya nuna adadin man mai da ba sa canzawa,coumarins, kumaflavonol glycosidesda kuma gungun wasu da ba a tantance baaglyconesdagaA. capillaris(Komiya et al., 1976,Yamahara et al., 1989). The muhimmanci man naA. capillaris(AEO) yana ɗaya daga cikin manyan magunguna masu aiki da magunguna kuma yana ba da anti-mai kumburi.Cha et al., 2009ada kuma anti-apoptotic Properties (Cha et al., 2009b). Duk da haka, kamar yadda AEO yana daya daga cikin manyan mahadi naA. capillaris, yuwuwar ayyukan hepatoprotective na manyan abubuwa dagaA. capillarisyakamata a bincika.

A cikin wannan binciken, tasirin kariya na AEO akancarbon tetrachloride(CCl4) - haifar dahepatotoxicityAn kimanta ta hanyoyin biochemical, kamar hantarage glutathione(GSH),malondialdehydeMatakan (MDA),superoxide dismutase(SOD), kumaglutathione peroxidase(GSH-Px) aiki, da kuma ayyukan naaspartate aminotransferase(AST) kumaalanine aminotransferase(ALT) a cikin jini. Hakanan an yi nazarin girman raunin hanta da aka haifar da CCl4 ta hanyar abubuwan lura na tarihi, tare da nazarin phytochemical ta GC-MS don gano abubuwan da ke cikin AEO.